《629_ftp》.pdf

TRANSLATIONAL AND CLINICAL RESEARCH Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Stem Cells: Let’s Not Get Lost in Translation JEFFREY M. GIMBLE,a BRUCE A. BUNNELL,b ERNEST S. CHIU,c FARSHID GUILAKd aStem Cell Biology Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA; bCenter for Stem Cell Research and Regenerative Medicine and cDivision of Plastic and Reconstructive Surgery, Department of Surgery, Tulane University Medical Center, New Orleans, Louisiana, USA; dOrthopaedic Research Laboratory, Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA Key Words. Adipose • Adipogenesis • Adult stem cells • Cellular therapy • Stem cell transplantation • Stromal cells • Clinical translation • Current Good Manufacturing Practices ABSTRACT Subcutaneous fat has emerged as an alternative tissue fall outside of traditional ‘‘hypothesis-driven, mechanism- source for stromal/stem cells in regenerative medicine. based’’ paradigm. This concise review examines the next Over the past decade, international research efforts have stages of the development process for therapeutic appli- established a wealth of basic science and preclinical evi- cations of adipose-derived cells and highlights the current dence regarding the differentiation potential and regener- state of the art regarding clinical trials. It is recom- ative properties of both freshly processed, heterogeneous mended that the experiments addressing these issues be stromal vascular fraction cells and culture expanded, reported comprehensively in the peer-review literature. relatively homogeneous adipose-derived stromal/stem This