《Use of Real-Time》.pdf

ARTICLE /ac Use of Real-Time, Label-Free Analysis in Revealing Low-Affinity Binding to Blood Group Antigens by Helicobacter pylori † ^ ^ || || § ‡ ,||,^ Y. Y. Fei, A. Schmidt, G. Bylund, D. X. Johansson, S. Henriksson, C. Lebrilla, J. V. Solnick, T. Boren,* and X. D. Zhu*,† †Department of Physics, ‡Departments of Medicine and Microbiology and Immunology, Center for Comparative Medicine, and §Department of Chemistry, University of California, Davis, Davis, California 95616, United States ) Department of Medical Biochemistry and Biophysics, Umea University, SE-90187 Umea, Sweden ^ Helicure AB, c/o Umea Biotech Incubator, Box 7997, Umea, Sweden S Supporting Information b ABSTRACT: Infectious diseases are often initiated by micro- bial adherence that is mediated by the binding of attachment molecules, termed adhesins, to cell surface receptors on host cells. We present an experimental system, oblique-incidence reflectivity difference (OI-RD) microscopy, which allows the detection of novel, low-affinity microbial attachment mechan- isms that may be essential for infectious processes. OI-RD microscopy was used to analyze direct binding of the onco- pathogen, Helicobacter pylori (H. pylori) to immobilized glyco- conjugates in real time with no need for labeling tags. The