柿叶黄酮的大孔吸附树脂分离提纯富集.pdfVIP

2005 年 3 月 中 成 药 March 2005 第 27 卷 　第 3 期 Chinese Traditional Patent Medicine Vol . 27 　No . 3 [制 　剂] 柿叶黄酮的大孔吸附树脂分离提纯富集 贝伟剑1 ,2 , 　彭文烈1 , 　罗 　杰2 ( 1. 中山大学生命科学学院 ,广东 广州 510275 ;2 . 广州白云山中药厂 ,广东 广州 510515) 关键词 :柿叶;黄酮 ;大孔树脂吸附;聚酰胺 ;富集 摘要 : 目的:研究 8 种不同类型吸附树脂对柿叶黄酮的吸附特性 。方法 :测试它们对柿叶黄酮的吸附率 、吸附速率 、解吸 率和产物得率及其黄酮含量 。结果 :发现聚酰胺和 AB8 树脂对柿叶黄酮吸附量大 ,吸附快 、解吸容易 ,吸附后产物黄酮 含量高 。结论 :聚酰胺和 AB8 树脂是一种性能良好的柿叶黄酮吸附剂 ,可用于从柿叶提取物中富集制备含量达 70 % 以 上的柿叶黄酮 ,为柿叶黄酮的开发提供新工艺 。 ( ) 中图分类号 :R284 . 2 　　　　　文献标识码 :A 　　　　　文章编号 2005 Enrichment of flavonoids from leave of Diospryr os kaki Linn ( Thunb) by macrore ticular resin ’s absorption 1 ,2 1 2 BEI Weij ian , 　PEN G Wenlie , 　LOU Jie ( 1. School of L if e Sciences , Zhongshan ( S un Yatsen) University , Guangzhou 510275 , China ; 2 . B ai YunShan TCM Pharmaceutical Factory , Guangzhou 510515 , China) KEY WORDS : Diospy ros kaki Linn ( Thunb . ) ; flavonoid ; macroreticular resin ’s absorption ; polyamide ; accumulation ABSTRACT : AIM : To screen macroreticular resins for isolation and purification of flavonoids from the leaves of Diospy ros ( ) kaki Linn FLDK . METHODS : 8 kinds of macroreticular resins were assayed for their adsorbability and deadsorbability as well as the adsorption kinetics to FLDK. RESUL TS : Polyamide and AB8 resin were found with good adsorbability and deadsorbability as well as adsorption kinetics to FLDK. CONCL USION : AB8 resin and polyamide could be used for pre paring high contents of FLDK. FLDKP70 , a flavonoid extract contained more than 77 % of flavonoids was prepared from the extract of leaves of Diospy ros kaki by polyamide adsorption column i