超声联合SonoVue介导基因转染治疗卵巢癌的实验的研究.pdf

目 录 一、摘要 中文论著摘要··············…··························“········1 英文论著摘要·····················································5 二、英文缩略语···············…···…·······························lo 三、论文 论文一、超声联合SonoVue介导基因GFP转染体内实验条件的优化 前一言··········“·····································”····“·····11月U舌”·””···“““””··””””““”．．”·”““””‘’”““。‘。 材料与方法·················………····························12 实验结果·························································16 讨论······························································22 结论······························································26 究 前。言·······················……···········……···········…··27日U舌”··一”·”。。”。‘一””……“”“”””…一“”。””。·…‘。27 材料与方法·········………···············…·············…··28 实验结果·······································…···············33 讨论······························································40 结论······························································47 四、本研究创新性的自我评价·······································48 五、参考文献····················································…··49 六、附录 绢；j盔······························································54 在学期间科研成绩···············································68 致谢………·········………………··············…··……·69 个人简介···············································…·······70 ·中文论著摘要· 超声联合SonoVue介导基因转染治疗 卵巢癌的实验研究 刖舌 肿瘤的生长通常伴有新生血管的形成。因此阻断新生血管的形成尤其是靶向 血管基因治疗已成为肿瘤治疗的发展方向之一。然而基因治疗成功的关键在于找 到一种安全、操作简便、转染效率高的基因携带载体。近年来，超声联合微泡造 影剂作为一种新型非病毒载体受到广泛关注。超声联合微泡造影剂增强基因转染 的前提是不损伤组织和器官，因此合适的辐照条件是非常重要的，但目前尚无定 论。 HSV．TK自杀基因系统。本研究通过裸鼠卵巢癌皮下移植瘤模型，探讨不同超声 机械指数(MI)、辐照时间及质粒浓度对体内基因转染效率的影响，优化超声联合 SonoVue介导基因转染的体内实验条件，提高基因转染的效率。进一步明确在此 最优实验条件下超声联合SonoVue介导KDR．TK基因转染治疗小鼠卵巢癌的有效 性，为卵巢癌靶向血管基因治疗提供一种新的基因导入方法。 材料与方法 l、利用人卵巢癌SK．OV-3细胞系构建裸鼠皮下移植瘤模型。