《Mesoporous Fe2O3 microspheres_phosphopeptides_MALDI-TOF MS》.pdfVIP

Journal of Colloid and Interface Science 318 (2008) 315–321 /locate/jcis Mesoporous Fe O microspheres: Rapid and effective enrichment 2 3 of phosphopeptides for MALDI-TOF MS analysis Lu Han 1, Zhe Shan 1, Dehong Chen, Xijuan Yu, Pengyuan Yang ∗ , Bo Tu, Dongyuan Zhao ∗ Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, PR China Received 26 July 2007; accepted 16 October 2007 Available online 22 October 2007 Abstract Mesoporous Fe2O3 microspheres have been successfully synthesized by the polymerization (urea and formaldehyde)-induced ferric hydroxide colloid aggregation. The urea-formaldehyde resin was removed by calcination in air. The obtained mesoporous Fe2O3 materials have spherical morphology with uniform particle size of ∼3.0 µm and porous surface with large inter-particle pores of ∼48.0 nm. The surface area is as large 2 3 as ∼33.3 m /g and the pore volume is 0.31 cm /g. The mesoporous Fe O microspheres were used for the enrichment of phosphopeptides for 2 3 the first time, in which high sensitivity, selectivity and capacity of specifically enriched phosphopeptides were achieved under a mild condition in a relative short time. After enriched from tryptic digest products of β-casein by the novel mesoporous Fe2O3 microspheres, phosphopeptides can be selectively detected with high intensity in MALDI-TOF