沙冬青种质资源抗旱基因研究.pdfVIP

缀臆组分黧分子功麓进行分类。并根据GO功麓富集分析，选择了响 应渗透胁迫、氧化胁迫、激素刺激和光信号刺激等4类共计27个抗 滤沙冬青撮为材料分析了表达模式，各塞响应酶方式不同。以lh和 就纛胁遗处理皆为上调表达且裙对表达量必2以上为标准确定为干旱 胁遗诱导酶基因，共计获得了4个基圜，分别编码糖苷永解酶家族蟹 融、过氧化物酶超家旗蛋白、谷胱甘肽过氧化物酶(㈣)和向光 反应性NPH3家族蛋国；以lh和72h胁迫处理誉为下调表达且相对 淡达量淹0．8戳下为标准确定炎干旱蓊追掷制酶基因，共计获褥了4 个基箧，分雾l编码麟永素家族蛋自(EPd914)、硫酸盐转运予 深入开展沙冬青抗旱机瑷积抗旱基因的研究夔定了基础。 美键谰： 沙冬青，干旱胁迫，抗氧化酶，转录缀，qRT-PCR ABSTRACT the broad—leafshrubinthenorthwestemdesertof As onlyevergreen shows resistanceto China，Ammopiptanthusmongolicusverystrong andcold。Inorderto the andmolecular drought explorephysical in mechanisms the tolerance underlyingdrought andbiochemicalcharacteristicsin analyzedphysiological A．mongolicus under stress。We seedlingdrought performedlarge—scale of root Roche／454 A。mongolicususing sequencing the ofA． sequencingtechnology；anddroughtresponsivegenes under treatmentwere mongolicusdifferentiallyexpresseddrought identifiedfurther real-timePCR resultsare by quantitative analysis．The discussedasfollow： The andRELintheleafof did RWC，MDA seedling A。mongolicus withcontrolundertreatmentof for not 20％PEG6000 changecompared 1h一72h．After24h oftherootof droughtstress，RWC A．mongolicus decreased