LBP对LPS刺激巨噬细胞分泌细胞因子调节作用.pdfVIP

20 10 8 1 35 8 M ed J Chin P LA , V o 35, N o 8, A ugust 1, 20 10 ! 937 ! LBP 对LPS刺激巨噬细胞分泌细胞因子的调节作用 舒旷怡, 张颖, 杨锦红, 任凭, 杨爱平,余玲玲, 李向阳 [ ] ( LBP) ( LPS) TH P1 , L BP LPS , L PS T HP1 , PMA , 4 : L PS ( LPS ) LBP ( L BP ) LBP/ L PS ( LBP L PS , 37 15min) L BP/ LPS ( LPS, LBP) 41224h , , T NF, IL10 IL6 , TH P1 , PM A , 1101001000ng/ m LPS T NF, 1ng/ m ( P 005) , 3 ( P 005) LBP , LBP 1000ng/ m , T NFIL6 LBP/ LPS , T NFIL6 LBP/ LPS 10 LBP/ LPS TN FIL6 , L PS 1000ng/ m IL10 8 0pg/ m , IL10 5pg/ m , LPS L BP ( F= 3425, P 001) , ( F = 4240, P = 0026) , L BP ( F = 4896, P 001) LPS , LPS , LBP ( P 005) LPS , L PS LBP , LBP LPS , LBP PMA , L PS , IL10 , [ ] ; ; ; 10; 6 [ ] R3495 [ ] A [ ] 2010) Regulation effects of LBP on activating macrophages to secrete cytokines * SHU Kuangyi, ZH ANG Y ing, YANG Jinhong, REN Ping, YAN G Aiping, YU Ling ing, LI Xiangyang . Depart ment of Inspection, Second Affi iat ed Hospit a of Wenzhou Medica Co ege, Wenzhou, Zhejiang 325027, China * Corresponding aut hor, Emai : xy@ wzhea thcom [Abstract] Objective To study the reg u atory effects of ipopo ysaccharide ( LPS) binding protein ( L BP) on the process of LPS transference and activation eff ects, and observe the secretion ba ance of macrophage ce s in secreting proinf ammatory cytokines and anti inf ammatory cytokines as a resu t of stimu ation by L PS . Metho s After pro iferation and passag e, the human T HP1 ce s w ere induced into macrophag es by PM A. Experiment w as divided into four groups: LPS group, L BP gr oup, LBP/ L PS preinc