GDNF与IL1体外诱导小鼠中脑神经干细胞分化作用的实验研究.pdfVIP

15 2 V ol . 15. No . 2 2006 4 CHINESE JOURNAL OF HISTOCHEMISTRYAND CYTOCHEMISTRY A pr. 200 6 GDN F IL1 * 黄 娟 戴冀斌 陆蔚天 赵涓涓 彭超华 , 4 3007 1 , , G DN F, IL1 GDN F + IL1 , T H , T H G DN F, IL1 T H 1341% , 92 3% , 1559 % , ( 34 9% , P ! 001) , GD N F G DN F, IL1 GDN F IL1 1 R3228 11 A EXPERIMENTAL STUDYON DIFFERENTIATION OF MOUSE MESENCEPHALIC NEURAL STEM CELLS INDUCED BY GDNFAND IL1In vitro * H u ang Ju an, Dai J ibin , Lu Weit ian , Zh ao J uanju an, P eng Chaohu a (Dep artment of H istology and Embryology , M edical School of Wuhan Univ ersity , Wuhan 4 3007 1, China) Abstract Object ive T o invest igat e t he f act ors f or t he diff erent iat ion o f mesenceph alic neural st em cells ( MN SCs) int o dopaminergic ( DA ergic) neuron s in v it ro, and t o prov ide t he b asic ex perimental infor m at ion f or t he therapy of P ar kin son s disease by cel l t ransplant at ion . Met hods MN SCs w ere cultured and pro liferat ed , t hen GDN F , IL1and G DN F + IL1w ere u sed t o indu ce diff er ent iat ion in t he condit ion of fet al bovine serum ( FBS) . Im munocyt ochemical t echniqu es w ere u sed t o det ect t he T Hir cells f rom N SC dif ferent iation , and F CM to det ect t he p ercent age of T Hir in all the cells . R esults T he proport ion s