英-GSPE对心脏缺血再灌注的作用.pdfVIP

J Mol Cell Cardiol 31, 1289–1297 (1999) Article No. jmcc.1999.0961, available online at on Cardioprotective Effects of Grape Seed Proanthocyanidin Against Ischemic Reperfusion Injury Motoaki Sato, Gautam Maulik, Partha S. Ray, Debasis Bagchi and Dipak K. Das University of Connecticut School of Medicine, Farmington, Connecticut; Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts and Creighton University School of Pharmacy and Allied Health Professions, Omaha, Nebraska, USA (Received 23 November 1998, accepted in revised form 24 March 1999) M. S, G. M, P. S. R, D. B D. K. D. Cardioprotective Effects of Grape Seed Proanthocyanidin Against Ischemic Reperfusion Injury. Journal of Molecular and Cellular Cardiology (1999) 31, 1289–1297. There is increasing evidence to indicate cardioprotective effects of red wine consumption. Such cardioprotective properties of wine have been attributed to certain polyphenolic constituents of grapes. The purpose of this investigation was to examine whether proanthocyanidins derived from grape seeds possess cardioprotective properties. Rats were randomly divided into two groups: grape-seed proanthocyanidin was administered orally to one group of rats (100 mg/kg/day) for 3 weeks while the other group served as control. After 3 weeks, rats were killed, hearts excised, mounted on the perfusion apparatus and perfused with Krebs–Henseleit bicarbonate (KHB) buffer. After stabilization hearts were perfused in the working mode for baseline measurements of contractile functions. Hearts were then subjected to 30 min of global ischemia followed by 2 h of reperfusion. Coronary perfusates were collected to monitor malonaldehyde formation, a presumptive marker for oxidative stress development. At the en