PCRDGGE技术分析塔式蚯蚓生态滤池微生物群落结构.pdfVIP

中国环境科学 2011,31(4) ：597~602 China Environmental Science PCR-DGGE 技术分析塔式蚯蚓生态滤池微生物群落结构 郭飞宏 1,郑 正 2*,张继彪 2 (1.南京大学环境学院,污染控制与资源化研究国家重点实验室,江苏 南京 210093 ； 2.复旦大学环境科学与工程系,上海 200433) 摘要：利用 PCR-DGGE 技术研究塔式蚯蚓生态滤池微生物群落结构变化,结果表明,微生物的变化与滤池体积大小、水力停留时间和污染 物负荷有关,微生物群落结构在不同滤池层中变化较 .12 个样品泳道中的总细菌的 Shannon-Wiener 多样性指数从 0.25 变化到 1.27,一级 滤池微生物多样性指数从 0.29 变化到 1.05,二级滤池微生物多样性指数从 0.75 变化到 0.97,三级滤池微生物多样性指数先从 0.87 减少到 0.25,后由于葡萄糖的加入增加到 1.27.二级滤池出水中葡萄糖的加入,加强了系统内微生物的活性,从而三级滤池砂石和青石层细菌条带变 亮变多.经过克隆测序和系统发育树分析,塔式蚯蚓生态滤池内的微生物多为单细胞菌属、假单细胞菌属、革兰氏阴性菌属和不可培养杆 菌属,不同细菌条带在不同级滤池内差异变化明显. 关键词：PCR-DGGE ；蚯蚓生态滤池；微生物群落；指数；菌属 中图分类号：X705 文献标识码：A 文章编号：1000-6923(2011)04-0597-06 1 Research on microbe community in tower earthworm ecology-filter by PCR-DGGE. GUO Fei-hong , ZHENG Zheng2* 2 , ZHANG Ji-biao (1.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China ；2.Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China). China Environmental Science, 2011,31(4)：597~602 Abstract ：Change of microbe community in tower earthworm ecology-filter was studied in this research with PCR-DGGE technology (polymerase chain reaction-denaturing gradient gel electrophoresis). These results showed that: the change of microbe community was obvious and linked with hydraulic resident time、pollutation burden and the volume of filters. Meanwhile, Shannon-Wiener Index of biodiversity increased from 0.25 to 1.27 in an all-round way, including 0.29～1.05 in the first filter, 0.75~0.97 in the second filter and 0.87~0.25~1.27 caused by some glucose added into influent in the last filter. Under the same reason, the activity and light of denitrifying bacteria were in