大孔吸附树脂法纯化菝葜中皂苷类成分和抑菌活性表征研究.pdfVIP

38 食品与药品 Food and Drug 2012年第 14卷第01期 大孔吸附树脂法纯化菝葜中皂苷类成分及抑菌活性表征研究 1 2 1* 周祥敏 ，张 辉 ，张 毅 （1. 重庆市食品药品检验所，重庆400015；2. 山东省生物药物研究院，山东济南 250014 ） 摘 要：目的 制备菝葜中抑菌活性部位。方法 以菝葜中皂苷类成分的洗脱率、精制度为指标，考察大孔吸附树脂对其皂 苷类成分的吸附性能和洗脱参数，并采用管碟法评测各洗脱部位的抑菌活性强度，以确证菝葜中皂苷类成分制备工艺的 合理性。结果 14.2 mL菝葜皂苷类成分样品液 （生药0.02 g/mL ）上大孔吸附树脂柱 （Φ15 mm ×H90 mm ，干重2.5g ）， 用蒸馏水、30 % 乙醇、70 % 乙醇各3 BV （柱床体积）依次洗脱，抑菌活性部位菝葜皂苷富集于70 % 乙醇洗脱液中。结 论 通过大孔吸附树脂富集与纯化，菝葜皂苷洗脱率为77.0 %，精制度为266.6 %，为开发菝葜抑菌活性部位提供了物质保 障，亦为研究其它皂苷类化合物的纯化工艺提供了实验参考。 关键词：大孔吸附树脂；纯化；菝葜皂苷；抑菌活性 中图分类号：R282.71 文献标识码：A 文章编号：1672-979X （2012 ）01-0038-05 Purification of Bamboo Brier Saponins by Macroporous Adsorption Resin and Its Bacteriostasis 1 2 1 ZHOU Xiang-min , ZHANG Hui , ZHANG Yi (1. Chongqing Institutef or Food and Drug Control, Chongqing 400015, China; 2. Institute of Biop harmaceuticals of Shandong Province, Jinan 250014, China) Abstract: Objective To prepare the antibacterial active site of bamboo brier. Methods With the elution rate and purity of bamboo brier saponins as the indications, the adsorption characteristics and elution parameters were investigated in the purification process of bamboo brier saponins by macroporous adsorption resin. The antibacterial activities of different elution fractions were measured by the cylinder-plate method in order to identify the rationality of preparation process of bamboo brier saponins. Results A 14.2 mL sample of bamboo brier saponins (0.02 g/mL crude drugs) was purified with a column of macroporous adsorption resin （Φ15 mm ×H90 mm ，dry weight 2.5 g ） and eluted with 3BV of distilled water, 30% ethanol and 70% ethanol, successively. Most of saponins were colle