Influence+of+Iron+Supplementation+on+DMT1+(IRE)-induced+Transport+of+Lead+by+Brain+Barrier+Systems+in+vivo.pdfVIP

Biomed Environ Sci, 2015; 28(9): 651-659 651 Original Article Influence of Iron Supplementation on DMT1 (IRE)-induced Transport of Lead by Brain Barrier Systems in vivo* 1,^ 2,^ 3,^ 1 AN Dai Zhi , AI Jun Tao , FANG Hong Juan , SUN Ru Bao , 1 1 1,# SHI Yun , WANG Li Li , and WANG Qiang 1. Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; 2. Beijing Vocational College of Agriculture, Beijing 102442, China; 3. Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China Abstract Objective To i nvestigate the potential involvement of DMT1 (IRE) protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the mRNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels.