Isolation+and+characterisation+of+human+gingival+margin-derived+STRO-1-MACS1+and+MACS2+cell+populations.pdfVIP

International Journal of Oral Science (2015) 7, 80–88 OPEN 2015 WCSS. All rights reserved 1674-2818/15 /ijos ORIGINAL ARTICLE Isolation and characterisation of human gingival margin-derived STRO-1/MACS1 and MACS2 cell populations 1,2 3 1 4 1 Karim M Fawzy El-Sayed , Sebastian Paris , Christian Graetz , Neemat Kassem , Mohamed Mekhemar , 5 ¨ 5 ¨ 1 Hendrick Ungefroren , Fred Fandrich and Christof Dorfer Recently, gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study’s aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS1) and STRO-1-negative (MACS2) cell populations from the human free gingival margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS1 and MACS2 cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS1 and MACS2 cell fractions showed plastic adherence. MACS1 cells, in contrast to MACS2 cells, showed all of t