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MiR-21调控雌激素缺乏导致的小鼠骨质疏松BMSCs成骨能力的研究.pdf
2015 9 (6 3 ) Orai Biomedicine ,Voi.6 ,No.3,Sep.2015 · 137·
[] 1674-8603(2015)03-0137-06
MiR-21
BMSCs
1 2 3 * 4 *
, , ,
(1.91551 ,332005 ;2.309 , 100091;3.
,,710032;4.
,, 710032)
[] :(mouse bone marrow stromai stem ceiis ,
mBMSCs)miRNA,miRNA mBMSCs
。 :,miRNA
C57BL/6J mBMSCs ,mBMSCs miRNA;RT-PCR
miRNA mBMSCs ; miRNA, RT-
PCR、Western biot、mBMSCs 。 :miRNA
mBMSCs miRNA miR-21;RT-PCR miR-21 mBMSCs
;miR-21 mBMSCs,。 :miR-21
mBMSCs miRNA;miR-21 mBM-
SCs 。
[] ;;miR-21;;
[] R780.2 [] A [doi] 10.3969/j.issn.1674-8603.2015.03.005
miR-21 regulates mBMSCs osteogenesis ability in estrogen deficiency-induced osteoporosis
1 2 3 4
WANG Guang ,YANG Nan ,DING Yin ,JIN Yan .(1.Dep artment of Out-p atient ,The PLA 91551th Troops ,Jiuj iang332005 ,China ;
2 .Dep artment of Stomatology ,The PLA 309th Hosp ital ,Beij ing100091 ,China ;3 .State Key Laboratory of Military Stomatology ,Dep art-
ment of Orthodontics ,School of Stomatology ,The Fourth Military Medical University ,Xi′an 710032 ,China ;4 .State Key Laboratory of
Military Stomatology ,Centre f or Tissue Engineering of Stomatology ,The Fourth Military Medical University ,Xi ′an 710032 ,China )
Corresp onding author :JIN Yan ,Email :yanj in@f mmu.edu.cn ,Tel :0086-29;DING Yin ,Email :dingyin@f mmu.edu.cn ,Tel :
0086-29
[Abstract ] Objective :To iooh for the hey miRNA that reguiates mouse bone marrow stromai stem ceiis (mBMSCs) osteogenesis in
the microenviroment of estrogen-deficiency-induced osteoporosis.To investigate the invoivement of hey miRNA in the osteogenic differ -
entiation of mBMSCs and the effect in this progress in estrogen -deficiency-induced osteoporosis.Methods :An ovariectomized animai
modei was empioye
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