Optimal+concentration+and+time+window+for+proliferation+and+differentiation+of+neural+stem+cells+from+embryonic+cerebral+cortex：5%+oxygen+preconditioning+for+72+hours.pdf

NEURAL REGENERATION RESEARCH September 2015,Volume 10,Issue 9 RESEARCH ARTICLE Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours 1, 2, * 2, * 3 2 Li-li Yuan , Ying-jun Guan , Deng-dian Ma , Hong-mei Du 1 Department of Histology and Embryology, Academy of Basic Medicine, Jining Medical University, Jining, Shandong Province, China 2 Department of Histology and Embryology, Academy of Basic Medicine, Weifang Medical University, Weifang, Shandong Province, China 3 Department of Otorhinolaryngology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, China Abstract *Correspondence to: Hypoxia promotes proliferation and differentiation of neural stem cells from embryonic day 12 Li-li Yuan or Ying-jun Guan, Ph.D., rat brain tissue, but the concentration and time of hypoxic preconditioning are controversial. To liliyuan06@126.com or address this, we cultured neural stem cells isolated from embryonic day 14 rat cerebral cortex in guanyj@. 5% and 10% oxygen in vitro. MTT assay, neurosphere number, and immunofluorescent staining found that 5% or 10% oxygen preconditioning for 72 hours improved neural stem cell viability orcid: and proliferation. With prolonged hypoxic duration (120 hours), the proportion of apoptotic 0000-0002-3689-7847 (Li-li Yuan) cells increased. Thus, 5% oxygen preconditioning for 72 hours promotes neural stem cell prolif- 0000-0002-