Optimal+concentration+and+time+window+for+proliferation+and+differentiation+of+neural+stem+cells+from+embryonic+cerebral+cortex:5%+oxygen+preconditioning+for+72+hours.pdf
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Optimal+concentration+and+time+window+for+proliferation+and+differentiation+of+neural+stem+cells+from+embryonic+cerebral+cortex:5%+oxygen+preconditioning+for+72+hours.pdf
NEURAL REGENERATION RESEARCH
September 2015,Volume 10,Issue 9
RESEARCH ARTICLE
Optimal concentration and time window for
proliferation and differentiation of neural stem
cells from embryonic cerebral cortex: 5% oxygen
preconditioning for 72 hours
1, 2, * 2, * 3 2
Li-li Yuan , Ying-jun Guan , Deng-dian Ma , Hong-mei Du
1 Department of Histology and Embryology, Academy of Basic Medicine, Jining Medical University, Jining, Shandong Province, China
2 Department of Histology and Embryology, Academy of Basic Medicine, Weifang Medical University, Weifang, Shandong Province, China
3 Department of Otorhinolaryngology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, China
Abstract
*Correspondence to: Hypoxia promotes proliferation and differentiation of neural stem cells from embryonic day 12
Li-li Yuan or Ying-jun Guan, Ph.D., rat brain tissue, but the concentration and time of hypoxic preconditioning are controversial. To
liliyuan06@126.com or address this, we cultured neural stem cells isolated from embryonic day 14 rat cerebral cortex in
guanyj@. 5% and 10% oxygen in vitro. MTT assay, neurosphere number, and immunofluorescent staining
found that 5% or 10% oxygen preconditioning for 72 hours improved neural stem cell viability
orcid: and proliferation. With prolonged hypoxic duration (120 hours), the proportion of apoptotic
0000-0002-3689-7847 (Li-li Yuan) cells increased. Thus, 5% oxygen preconditioning for 72 hours promotes neural stem cell prolif-
0000-0002-
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