PAX3对靶基因MITF转录活性调控的实验研究.pdf

P A X M I T F air box PAXmicrophthalmia asso p ciated transcription factor M IT F I Waardenburg Waardenburg syndrome WS PAX HD Hf sX T M IT F PAX M IT F PAX attaat DNA PAXHD Hf sX PAX M IT F HD M IT F PAX P Hf sX M IT F P PAX P HD PAX M IT F DNA attaat Hf sX HD Hf sX M IT F I WS Waardenburg DOI issn j http w w w cnki net kcms detail R html R A Experimental Studies on Regulation of PAX on Transcriptional Activities of Target Gene MITF Zhang Hua Chang Shangkui Feng Yong Qian Minf ei Li Jiping Zhang Chun Department of Otorhinolaryngology Renj i Hospital Shanghai Jiaotong University School of Medicine Shanghai China Abstract Obj ective T o investigate the impact of pair box PAX ene mutations on transcriptional ac g tivity of target gene microphthalmia associated transcription f actor M IT F and the role it play s in the pathogene sis of Waardenburg syndrome type I Methods T he T cells w ere transient transfected w ith w ild type WT PAX and mutant type M T HD Hf sX lasmids We observed and analy sed the regulation eff ects of WT p M T PAX on the transcriptional activities of M IT F and the influence of the tw o mutants on WT PAX f unction u sing luciferase activity assay s detect DNA binding capacity of WT M T PAX to M IT F gene promoter using a bio ti nyl