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PAX3对靶基因MITF转录活性调控的实验研究.pdf
P A X M I T F
air box PAXmicrophthalmia asso
p
ciated transcription factor M IT F I Waardenburg Waardenburg syndrome
WS PAX HD Hf sX T
M IT F PAX M IT F
PAX attaat DNA
PAXHD Hf sX PAX M IT F
HD M IT F PAX
P Hf sX M IT F P PAX
P HD PAX M IT F DNA
attaat Hf sX HD Hf sX M IT F
I WS
Waardenburg
DOI issn
j
http w w w cnki net kcms detail R html
R A
Experimental Studies on Regulation of PAX on Transcriptional Activities of Target Gene MITF
Zhang Hua Chang Shangkui Feng Yong Qian Minf ei Li Jiping Zhang Chun
Department of Otorhinolaryngology Renj i Hospital Shanghai Jiaotong
University School of Medicine Shanghai China
Abstract Obj ective T o investigate the impact of pair box PAX ene mutations on transcriptional ac
g
tivity of target gene microphthalmia associated transcription f actor M IT F and the role it play s in the pathogene
sis of Waardenburg syndrome type I Methods T he T cells w ere transient transfected w ith w ild type WT
PAX and mutant type M T HD Hf sX lasmids We observed and analy sed the regulation eff ects of WT
p
M T PAX on the transcriptional activities of M IT F and the influence of the tw o mutants on WT PAX f unction u
sing luciferase activity assay s detect DNA binding capacity of WT M T PAX to M IT F gene promoter using a bio ti
nyl
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