Polymeric+nanocomposites+loaded+with+lfuoridated+hydroxyapatite+Ln3++(Ln+=+Eu+or+Tb)-iron+oxide+for+magnetic+targeted+cellular+imaging.pdf

Cancer Biol Med 2015;12:175-183. doi: 10.7497/j.issn.2095-3941.2015.0014 ORIGINAL ARTICLE Polymeric nanocomposites loaded with fluoridated hydroxyapatite Ln3+ (Ln = Eu or Tb)/iron oxide for magnetic targeted cellular imaging 1 1 2 1 1 3 Jie Pan , Wei-Jiao Liu , Chao Hua , Li-Li Wang , Dong Wan , Jun-Bo Gong 1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China; 2 Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing 100190, China; 3 Department of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China ABSTRACT Objective: To fabricate polymeric nanocomposites with excellent photoluminescence, magnetic properties, and stability in aqueous solutions, in order to improve specificity and sensitivity of cellular imaging under a magnetic field. Methods: Fluoridated Ln3+-doped HAP (Ln3+-HAP) NPs and iron oxides (IOs) can be encapsulated with biocompatible polymers via a modified solvent exaction/evaporation technique to prepare polymeric nanocomposites with fluoridated Ln3+-HAP/iron oxide. The nanocomposites were characterized for surface morphology, fluorescence spectra, magnetic properties and in vitro cytotoxicity. Magnetic targeted cellular imaging of such nanocomposites was also evaluated with confocal laser scanning microscope using A549 cells with or without magnetic field. Results: The fabricated nanocomposites showed good stability and excellent luminescent properties, as well as low in vitro