Human+induced+pluripotent+stem+cells+labeled+with+lfuorescent+magnetic+nanoparticles+for+targeted+imaging+and+hyperthermia+therapy+for+gastric+cancer.pdf

Cancer Biol Med 2015;12:163-174. doi: 10.7497/j.issn.2095-3941.2015.0040 ORIGINAL ARTICLE Human induced pluripotent stem cells labeled with fluorescent magnetic nanoparticles for targeted imaging and hyperthermia therapy for gastric cancer 1 1 1 1 1 2 3 2 2 Chao Li , Jing Ruan , Meng Yang , Fei Pan , Guo Gao , Su Qu , You-Lan Shen , Yong-Jun Dang , Kan Wang , Wei-Lin 1 1 Jin , Da-Xiang Cui 1Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, Shanghai 200240, China; 2 Basic Medical Sciences Department of Biochemistry Molecular Biology Key Laboratory of Molecular Medicine, Fudan University, Shanghai 200032, China; 3Department of Imaging and ’ Nuclear Medicine, Shanghai Sixth People s Hospital, Shanghai 20006, China ABSTRACT Objective: Human induced pluripotent stem (iPS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human iPS cells labeled with fluorescent magnetic nanoparticles (FMNPs) for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods: Human iPS cells were prepared and cultured for 72 h. The culture medium was collected, and then was co- incubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human iPS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was