Probing the dynamics of growth factor receptor by single-molecule fluorescence imaging》.pdf

Progress in Biophysics and Molecular Biology 118 (2015) 95e102 Contents lists available at ScienceDirect Progress in Biophysics and Molecular Biology journal homepage: /locate/pbiomolbio Review Probing the dynamics of growth factor receptor by single-molecule fluorescence imaging * Yahong Sun, Nan Li, Xiaohong Fang Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, China a r t i c l e i n f o a b s t r a c t Article history: Single-molecule fluorescence imaging with living cells offers a new approach to visualize and monitor Available online 9 May 2015 individual proteins during their cellular activities. It facilities the study of cell signaling proteins whose structures and interactions are highly temporal and spatial regulated. In this review, we will mainly Keywords: present our recent work on probing the dynamics of two types of important transmembrane growth Single-molecule fluorescence imaging factor receptors, transforming growth factor b receptors and epidermal growth factor receptors, by Signal transduction single-molecule fluorescence microscopy. This includes the characterization of receptor stoichiometry, Dynamics