Protein kinase D2 promotes the proliferation of glioma cells by regulating Golgi phosphoprotein 3》.pdf

Cancer Letters 355 (2014) 121–129 Contents lists available at ScienceDirect Cancer Letters journal homepage: /locate/canlet Original Articles Protein kinase D2 promotes the proliferation of glioma cells by regulating Golgi phosphoprotein 3 Xiuping Zhou a,b,*,1, Pengfei Xue c,1, Minglin Yang c,1, Hengliang Shi a,b, Dong Lu a,b, Zhaohao Wang c, Qiong Shi a,b, Jinxia Hu a,b, Shao Xie a,b, Wenjian Zhan a,b, Rutong Yu a,b,** a Brain Hospital, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China b Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, Jiangsu, China c The Graduate School, Xuzhou Medical College, Xuzhou, Jiangsu, China A R T I C L E I N F O A B S T R A C T Article history: Protein kinase D2 (PKD2) has been demonstrated to promote tumorigenesis in many types of cancers. Received 12 July 2014 However, how PKD2 regulates cancer cell growth is largely unknown. In this study, we found that over- Received in revised form 3 September 2014 expression of PKD2 promoted glioma cell growth but down-regulation of PKD2 inhibited it. Further Accepted 4 September 2014 investigation indicated that PKD2 down-regulation decreased the protein level of Golgi phosphoprotein 3(GOLPH3) as well as p-AKT level. On the contrary, over-expression of PKD2 increased the protein level Keywords: of GOLPH3 and p-AKT. In addition, GOLPH3 exhibited similar effect on glioma cell growth to that of PKD2. Glioblastoma PKD2