Quantification of organic P and low-molecular-weight organic acids in ferralsol soil extracts by ion chromatography》.pdfVIP

Geoderma 257–258 (2015) 94–101 Contents lists available at ScienceDirect Geoderma jour nal homepage: www. elsevi er. com/ locate/ geoderma Quantification of organic P and low-molecular-weight organic acids in ferralsol soil extracts by ion chromatography Kittima Waithaisong a, Agnès Robin b, Agnès Martin b, Michael Clairotte a, Manon Villeneuve c, Claude Plassard a,⁎ a INRA, UMR EcoSols, 2 place Viala, 34060 Montpellier, Cedex 1, France b CIRAD, UMR EcoSols, 2 place Viala, 34060 Montpellier, Cedex 1, France c IRD, UMR EcoSols, 2 place Viala, 34060 Montpellier, Cedex 1, France a r t i c l e i n f o a b s t r a c t Article history: A better understanding of the composition of organic phosphorus (organic P) and low-molecular-weight organic Received 27 July 2014 acids (LMWOAs) in soil will make it possible to predict phosphorus cycling, improve biomass production and Received in revised form 26 December 2014 help phosphorus management in forest plantations. The method for quantifying organic P and LMWOAs in soil Accepted 29 December 2014 extracts was developed using ion chromatography (IC). Sodium hydroxide (0.5 N) was validated as an extractant Available online 5 January 2015 for both organic P and LMWOAs. A mixture of 200 mM NaOH and ultrapure water was used to create a linear gra- dient elution. The eluent program separated five different organic P forms (glucose-6-phosphate, AMP, fructose- Keyword