Quantification of plant cell wall monosaccharides by reversed-phase liquid chromatography with 2-aminobenzamide pre-column derivatization and a non-toxic reducing reagent 2-picoline borane》.pdf

Journal of Chromatography A, 1414 (2015) 122–128 Contents lists available at ScienceDirect Journal of Chromatography A j o u r n a l h o me p a g e : w w w. elsev /locate/chro ma Quantification of plant cell wall monosaccharides by reversed-phase liquid chromatography with 2-aminobenzamide pre-column derivatization and a non-toxic reducing reagent 2-picoline borane Jingjing Fang, Guochen Qin, Jun Ma, Yi-Min She ∗ Shanghai Centerfor Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602, China a r t i c l e i n f o a b s t r a c t Article history: In this report, we described a sensitive method for quantifying plant cell wall monosaccharides using Received 11 May 2015 chemical derivatization, reversed-phase high performance liquid chromatography separation with ultra- Received in revised form 8 August 2015 violet detection (HPLC-UV). Monosaccharides were derivatized with 2-aminobenzamide (2-AB) by Accepted 20 August 2015 reductive amination to increase the hydrophobicity and detected by ultraviolet absorption for HPLC- Available online 22 August 2015 UV analysis. A non-toxic reductant, 2-picoline borane was utilized to replace the traditionally used sodium cyanoborohydride (NaCNBH3 ) to avoid the formation of toxic by-products. Experimental con- Keywords: ditions were optimized using glucose as a model system to achieve a high reaction yield of 99%. Under Plant ce