Quantification of protein copy number in single mitochondria The Bcl-2 family proteins》.pdf

Biosensors and Bioelectronics 74 (2015) 476–482 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios Quantification of protein copy number in single mitochondria: The Bcl- 2 family proteins Chaoxiang Chen a, Xiang Zhang a, Shuyue Zhang a, Shaobin Zhu a, Jingyi Xu a, Yan Zheng a, Jinyan Han a, Jin-Zhang Zeng b, Xiaomei Yan a,n a The MOE Key Laboratory of Spectrochemical Analysis Instrumentation, The Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, PR China b School of Pharmaceutical Sciences and Institute for Biomedical Research, Xiamen University, PR China a r t i c l e i n f o a b s t r a c t Article history: Bcl-2 family proteins, represented by antiapoptotic protein Bcl-2 and proapoptotic protein Bax, are key Received 28 May 2015 regulators of mitochondria-mediated apoptosis pathway. To build a quantitative model of how Bcl-2 Received in revised form family protein interactions control mitochondrial outer membrane permeabilization and subsequent 23 June 2015 cytochrome c release, it is essential to know the number of proteins in individual mitochondria. Here, we Accepted 25 June 2015 report an effective method to quantify the copy number and distribution of proteins in single mi- Available online 3 July 2015