Quantitative and morphologic analysis on exopolysaccharide and biomass production from a truffle endophytic fungus Hypocreales sp. NCHU01》.pdf

Journal of the Taiwan Institute of Chemical Engineers 45 (2014) 108–114 Contents lists available at ScienceDirect Journal of the Taiwan Institute of Chemical Engineers jou r nal h o mep age: w ww.els evier .co m/lo c ate/jtic e Quantitative and morphologic analysis on exopolysaccharide and biomass production from a truffle endophytic fungus Hypocreales sp. NCHU01§ Chiung-Wen Yeh a, Chi-Zong Zang a, Chia-Chi Lin a, Shu-Chen Kan a, Wei-Feng Chang a, Chwen-Jen Shieh b, Yung-Chuan Liu a,* a Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC b Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC A R T I C L E I N F O A B S T R A C T Article history: In this study, exopolysaccharides (EPS) produced from Hypocreales sp. NCHU01 was carried out in the Received 29 October 2012 submerged cultivation. To find the optimal conditions, factors such as carbon and nitrogen source Received in revised form 24 September 2013 concentrations, pH, temperature, magnesium ion, and potassium ion levels were considered. The results Accepted 29 September 2013 showed that the optimal conditions were as follows: sucrose, yeast extract, magnesium sulfate and Available online 15 November 2013 potassium dihydrogen phosphate were 10.6 g/L, 10.92 g/L, 1.0 g/L and 1.01 g/L, respectively. The optimization conditions were set at 25 8C with an initi