Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol》.pdf

Analytica Chimica Acta 798 (2013) 82–88 Contents lists available at ScienceDirect Analytica Chimica Acta j o u r n a l h o m e p ag e: w w w. elsev /locate/aca Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol Xun Yao a,b , Panpan Yan a,b , Qinghui Tang a,b , Anping Deng a,b,∗ , Jianguo Li a,b,∗∗ a College of Chemistry, Chemical Engineering Materials Science, Soochow University, Suzhou 215123, China b The Key Lab of Health Chemistry Molecular Diagnosis of Suzhou, Suzhou 215123, China h i g h l i g h t s g r a p h i c a l a b s t r a c t • An ultrasensitive ECL immunosensor of CdSe QDs for clenbuterol determi- nation is developed. • The CdSe QDs showed great biocom- patibility and could be easier to make direct use of such QDs in the region of biological system. • Enzymatic amplification strategy was proposed by combining the coreac- tant and pAb/GaRIgG-HRP. • Enzymatic amplification increased ECL emission and extended the ana- lyte in presence of substrate. • It provided a method for detecting clenbuterol and enlarged the usage of QDs in ECL biosensing. a r t i c l e i n f o a b s t r a c t Article history: An ultrasensitive electrochemiluminescence (ECL) immunosensor based on CdSe quantum dots (QDs) Received 29 January 2013 has been designed for the detection of clenbuterol. The immunosensor was fabricated by layer by layer Received in revised form 15 July 2013 and characterized with atomic force microscopic images (AFM) and electrochemical impedance spec- Accepte