RAD59 is required for efficient repair of simultaneous double-strand breaks resulting in translocations in Saccharomyces cerevisiae》.pdf

d n a r e p a i r 7 ( 2 0 0 8 ) 788–800 a v a i la b le at ww w.scie ncedirect. com j o u rn al h omepage: www.elsevi er.c om /locate/dnarepair RAD59 is required for efficient repair of simultaneous double-strand breaks resulting in translocations in Saccharomyces cerevisiae Nicholas R. Pannunzio a,b, Glenn M. Manthey a, Adam M. Bailis a,∗ a Division of Molecular Biology, Beckman Research Institute of the City of Hope, 1500 E. Duarte Road, Duarte, CA 91010-0269, United States b City of Hope Graduate School of Biological Sciences, 1500 E. Duarte Road, Duarte, CA 91010-3000, United States a r t i c l e i n f o a b s t r a c t Article history: Exposure to ionizing radiation results in a variety of genome rearrangements that have Received 3 March 2007 been linked to tumor formation. Many of these rearrangements are thought to arise from Received in revised form the repair of double-strand breaks (DSBs) by several mechanisms, including homologous 25 January 2008 recombination (HR) between repetitive sequences dispersed throughout the genome. Doses Accepted 8 February 2008 of radiation sufficient to create DSBs in or near multiple repetitive elements simultane- Published on line 25 March 2008 ously could initiate single-strand annealing (SSA), a highly efficient, though mutagenic, mode of DSB repair. We have investigated the genetic control of the formation of transloca- Keywords: tions that occur spontaneously and those that form after the generation of DSBs adjacent