Raman Spectra Analysis for Single Mitochondrias after Apoptosis Process of Yeast Cells Stressed by Acetic Acid》.pdf

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY Volume 43, Issue 5, May 2015 Online English edition of the Chinese language journal Cite this article as: Chin J Anal Chem, 2015, 43(5), 643–650. RESEARCH PAPER Raman Spectra Analysis for Single Mitochondrias after Apoptosis Process of Yeast Cells Stressed by Acetic Acid 1,2 2 1,2 1 3 2, LI Bing , LU Ming-Qian , WANG Qiao-Zhen , SHI Gui-Yu , LIAO Wei , HUANG Shu-Shi * 1 College of Life Science, Guangxi Normal University, Guilin 541004, China 2 Biophysics Laboratory, Guangxi Academy of Sciences, Nanning 530007, China 3 Guangxi Vocational and Technical College, Nanning, 530226, China Abstract: Laser tweezers Raman spectroscopy (LTRS) as a non-invasive tool for mitochondria analysis, combined with oxygen electrode and ultraviolet spectrophotometric method, was used to investigate the Raman spectra of mitochondria of yeast cells in vitro and in vivo after induced with acetic acid. The results showed that when the mitochondria of yeast cells in vivo was induced by acetic acid, the spectral peaks of the nucleic acid (1081 and 1301 cm–1), proteins (872, 1604, 1445 and 1657 cm–1), lipids (1125, 1301, 1445 and 1657 cm–1 ), cytochrome c (750 and 1125 cm–1) and mitochondria respiration (1604 cm–1) were significantly decreased as a function of the duration of the acetic acid stress, and the obtained physiological and biochemical indexes of respiration rate, phosphorous/oxygen (P/O) ration and cytochrome c content were similar to those by conventional method. Furthermore, when the mitochondria of yeast cells in vitro was induced by acetic acid, the spectral peaks of the nucleic acid (1081 and 1301 cm