Redox regulation of CF1-ATPase involves interplay between the γ-subunit neck region and the turn region of the βDELSEED-loop》.pdf

Biochimica et Biophysica Acta 1847 (2015) 441–450 Contents lists available at ScienceDirect Biochimica et Biophysica Acta j ournal homepa ge: /l ocate/ bb abio Redox regulation of CF -ATPase involves interplay between the 1 γ-subunit neck region and the turn region of the βDELSEED-loop Felix Buchert a, Hiroki Konno a,b, Toru Hisabori a,c,⁎ a Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259-R1-8, Midori-ku, Yokohama 226-8503, Japan b Imaging Research Division, Bio-AFM Frontier Research Center, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan c Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo 102-0075, Japan a r t i c l e i n f o a b s t r a c t Article history: The soluble F complex of ATP synthase (F F ) is capable of ATP hydrolysis, accomplished by the minimum cat- 1 o 1 Received 6 November 2014 alytic core subunits α β γ. A special feature of cyanobacterial F and chloroplast F (CF ) is an amino acid se- 3 3 1 1 1 Received in revised form 23 December 2014 quence inserted in the γ-subunit. The insertion is extended slightly into the CF1 enzyme containing two Accepted 27 January 2015 additional