原创力文档Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis》.pdf
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Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis》.pdf
Developmental and Comparative Immunology 53 (2015) 47e54
Contents lists available at ScienceDirect
Developmental and Comparative Immunology
journal homepage: /locate/dci
Recombinant expression and functional analysis of an isoform of anti-
lipopolysaccharide factors (FcALF5) from Chinese shrimp
Fenneropenaeus chinensis
a, b, 1 a, c, 1 a, c, * a, b a
Hui Yang , Shihao Li , Fuhua Li , Xinjia Lv , Jianhai Xiang
a Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
b University of Chinese Academy of Sciences, Beijing 100049, China
c National Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao 266071, China
a r t i c l e i n f o a b s t r a c t
Article history: Antimicrobial peptides (AMPs) have a great potential to be used as a substitute for antibiotics since AMPs
Received 24 April 2015 dont lead to bacterias drug resistance. Anti-lipopolysaccharide factors (ALFs) are one type of AMPs and
Received in revised form exist in crustaceans. In the present study, we produced a recombinant protein (rFcALF5) of an ALF iso-
23 June 2015
form (FcALF5) from Chinese shrimp Fenneropenaeus chinensis through a prokaryotic expression system.
Accepted 24 June 2015
The rFcALF5 exhibited varied antibacterial activities against different bacteria. Besides its antibacterial
Available online 26 Ju
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