Redox regulation of metabolic and signaling pathways by thioredoxin and glutaredoxin in NOS-3 overexpressing hepatoblastoma cells》.pdf

Redox Biology 6 (2015) 122–134 Contents lists available at ScienceDirect Redox Biology journal homepage: /locate/redox Research Paper Redox regulation of metabolic and signaling pathways by thioredoxin and glutaredoxin in NOS-3 overexpressing hepatoblastoma cells Raúl González a,b,n, M. José López-Grueso a, Jordi Muntané c,d, J. Antonio Bárcena a,b, C. Alicia Padilla a,b a Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain b Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain c Departamento de Cirugía General, Hospital Universitario Virgen del Rocío/Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla, Sevilla, Spain d Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain a r t i c l e i n f o a b s t r a c t Article history: Nitric oxide (NO) plays relevant roles in signal transduction in physiopathology and its effects are de- Received 22 May 2015 pendent on several environmental factors. NO has both pro-apoptotic and anti-apoptotic functions but Received in revised form the molecular mechanisms responsible for these opposite effects are not fully understood. The action of 9 July 2015 NO occurs mainly through redox changes in target proteins, particularly by S-nitrosylation of reactive Accepted 14 July 2015 cysteine residues. Thioredoxin (Trx) and glutaredoxin (Grx) systems