Regulating myogenic differentiation of mesenchymal stem cells using thermosensitive hydrogels》.pdf

Acta Biomaterialia 26 (2015) 23–33 Contents lists available at ScienceDirect Acta Biomaterialia journal homepage: /lo cate/actabiomat Regulating myogenic differentiation of mesenchymal stem cells using thermosensitive hydrogels Yanyi Xu a,1, Zhenqing Li a,1, Xiaofei Li a, Zhaobo Fan a, Zhenguo Liu b, Xiaoyun Xie c, Jianjun Guan a,d,⇑ a Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, United States b Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, United States c Department of Gerontology, Tongji Hospital, Tongji University, Shanghai, China d Tongji Hospital, Tongji University, Shanghai, China a r t i c l e i n f o a b s t r a c t Article history: Stem cell therapy has potential to regenerate skeletal muscle tissue in ischemic limb. However, the deliv- Received 16 April 2015 ered stem cells experience low rate of myogenic differentiation. Employing injectable hydrogels as stem Received in revised form 30 July 2015 cell carriers may enhance the myogenic differentiation as their modulus may be tailored to induce the Accepted 11 August 2015 differentiation. Yet current approaches used to manipulate hydrogel modulus often simultaneously vary Available online 12 August 2015 other properties that also affect stem cell differentiation, such as chemical structure, composition and water content. Thus it is challenging to demon