Relationship between macrophages in mouse uteri and angiogenesis in endometrium during the peri-implantation period》.pdf

Theriogenology 82 (2014) 1021–1027 Contents lists available at ScienceDirect Theriogenology journal homepage: Relationship between macrophages in mouse uteri and angiogenesis in endometrium during the peri-implantation period 1 * Wenya Tan , Leining Chen, Lei Guo, Xianghong Ou, Duo Xie, Song Quan Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China a r t i c l e i n f o a b s t r a c t Article history: The objective of this study is to examine the change in macrophage numbers, inducible form of Received 16 December 2013 NO synthase (iNOS), and vascular endothelial growth factor (VEGF) expression both before and Received in revised form 11 July 2014 after embryo implantation in the uterine tissue of mice. In order to explore the mechanism of Accepted 15 July 2014 macrophages in endometrial angiogenesis, 8-week-old female mice were divided into three groups: pregnant group, pseudopregnant group (mated to male mice that had been vasecto- Keywords: mized), and estrous group (unmated). Individuals from these three groups were sacrificed at Macrophage time intervals D1.5 to D6.5. Formalin-fixed paraffin-embedded tissue was used for immuno- Induced nitric oxide synthase Vascular endothelial growth factor