椎间盘退变论文：MMP-1和TIMP-1在人退变椎间盘髓核中的表达.docVIP

椎间盘退变论文：MMP-1及TIMP-1在人退变椎间盘髓核中的表达 【中文摘要】椎间盘退变是引起下腰痛的重要原因之一,椎间盘退变是一个复杂的生理过程,其发生机制尚未完全阐明,近年来研究表明,椎间盘病变与基质代谢失衡有密切关系。本研究通过测定MMP-1及TIMP-1在人退变椎间盘髓核中的表达,探讨其在人椎间盘退变发生、发展中的作用。方法选取2009-2010年青海大学附属医院骨科腰椎间盘突出症手术切除髓核标本25例及外伤致胸腰椎骨折切除髓核15例作为对照组。采用免疫组化法检测退变椎间盘髓核细胞中MMP-1及TIMP-1表达。结果在椎间盘髓核细胞中MMP-1的表达实验组的阳性率高于对照组(p0.01);椎间盘髓核细胞中TIMP-1的表达病例组阳性率高于对照组(p0.01)。退变椎间盘髓核细胞有高表达的MMP-1,而TIMP-1增加甚少,MMP-1/TIMP-1失调。结论退变椎间盘髓核细胞中MMP-1及TIMP-1表达升高,退变椎间盘髓核细胞中MMP-1/TIMP-1失调,在椎间盘突出的发生、发展中发挥重要作用。进一步研究MMPs与TIMPs的调控机制,有助于阐明椎间盘退变发病机制,为椎间盘突出症的临床干预提供新的靶点。 【英文摘要】Disc degeneration is one of the important causes of low backpain,and which is a complex physiological process,its mechanism have not yet been fully clarified.The test is to determine the involvement of pathomeehanism of intervertebral disc degeneration by monitoring the inununohistoehemieally of MMP-1and TIMP-1 in nucleus pulposus cells of intervertebral disc degeneration.Methods Immunohistochemistry assay were used to detect the expression of MMP-1and TIMP-1 in Nucleus pulposus cells in 40 cases.From 2009 to 2010 , 25 cases with lumber disc herniation and 15 cases of thoracic-lumber broken were choosen.Results The expression of MMP-1 in degenerated discs was higher than the normal discs(P0.01).The expression of TIMP-1 in in degenerated discs was higher than the normal discs (P0.01).In the degenerative disc cell exhibited positive staining for MMP-1 is much higher than for TIMP-1.The balance of TIMP-1/MMP-1 is broken.Conclusions MMP-1 and TIMP-1 may be invovled in the pathogenesy of disc degeneration, they are very important in disc degeneration.The mechanism of MMPs and TIMPs in the future study may find out the reason of disc degeneration which will provide new treatment for lumber disc protrusion disease. 【关键词】椎间盘退变 基质金属蛋白酶-1 基质金属蛋白酶抑制剂-1 免疫组织化学 【英文关键词】disc degeneration MMP-1 TIMP-1 immunohistochemistry 【目录】MMP-1及TIMP-1在人退变椎间盘髓核中的表达 中文摘要 3-4 ABSTRACT 4-5 前言 6-9 第一章 材料与方法 9-13 1. 研究对象 9 2. 标本采集及处理 9 3. 检测方法及其原理 9 4. 主要试剂 9-10 5. 主要仪器