利用高通量测序技术发现植物小分子RNA及研究进展.pdfVIP

中国农业科学 2009,42(11):3755-3764 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.11.001 利用高通量测序技术发现植物小分子RNA研究进展 卫 波，张荣志，李爱丽，毛 龙 （中国农业科学院作物科学研究所/ 国家农作物基因资源与遗传改良重大科学工程，北京 100081 ） 摘要：小分子 RNA 是一类长约 20～30 个核苷酸的非编码 RNA 分子，其介导的转录后基因调控是植物中的一 种新型基因调控机制。它在植物的生长发育和适应外界各种环境胁迫的过程中起着非常重要的作用。植物中小分 子 RNA 数量巨大、种类繁多，而高通量测序技术的出现大大加快了它们的发现过程。本文在简要介绍目前已知植 物小分子 RNA 的类型及特点的基础上，综合阐述近年来利用高通量测序技术克隆和分析植物小分子 RNA 的研究成 果，以期反映当前植物小分子RNA的基因组分布规律、功能和进化等方面的最新研究进展。 关键词：高通量测序技术；454-FLX；Solexa；小分子RNA；微RNA；小干扰RNA Progress in Plant Small RNA Research via High-Throughput Sequencing WEI Bo, ZHANG Rong-zhi, LI Ai-li, MAO Long (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081) Abstract: Small RNAs are 20-30 nucleotides long non-coding RNAs. Small RNA-mediated post-transcriptional regulation represents a new mechanism of gene regulation and plays important roles in plant growth and development as well as responses to different environmental stresses. In plants, there are tremendous amount of small RNAs of various types. The advance of high- throughput sequencing technology has significantly facilitated their discoveries. In this review, in addition to a brief introduction of small RNA features, the authors attempted to summarize recent progresses in small RNA research using next generation sequencing technology and aim to highlight the new insights in their genome organization, function and evolution in plants. Key words: high-throughput sequencing; 454-FLX; Solexa; small RNA; microRNA; siRNA