三参汤治疗糖尿病肾病的实验的研究.pdfVIP

目 录 中文摘要 ···················································3 英文摘要 ···················································4 引 言 ····················································6 正 文 ····················································9 1 实验材料和方法 ·············································9 1.1 实验对象 ·················································9 1.2 实验方法················································10 2 实验指标的记录和测定······································10 2.1 实验取材················································11 2.2 实验检测················································11 3 实验结果与分析············································12 3.1 大鼠治疗前后体重、血糖、尿微量白蛋白变化情况 ··············12 3.2 Cystatin C含量测定 ·······································15 3.3 ET-1含量测定 ············································16 3.4ATII含量、血浆（肾组织）ACE活性和VEGF含量测定 ············16 4 实验结果讨论··············································18 4.1 中医对糖尿病肾病的认识··································18 4.2 三参汤的组方依据 ········································23 4.3 安博维的临床治疗作用 ····································26 4.4 糖尿病肾病大鼠模型的建立 ································27 4.5 实验指标分析 ············································28 5 存在的问题和展望 ··········································30 结 论 ··················································31 1 参考文献 ··················································32 综 述 ··················································36 致 谢 ··················································43 攻读学位期间发表的学术论文目录 ·······················44 作者声明 ··················································45 个人简历 ··················································46 2 中 文 摘 要 目 的：探讨三参汤治疗早期糖尿病肾病的作用机制。 方 法：利用高脂饮食+链脲佐菌素的方法诱发糖尿病肾病大鼠模型 （以血糖和尿微量白蛋白作为判定标准），以安博维为阳性对照，以尿 微量白蛋白、血清半胱氨酸蛋白酶抑制剂C等作为肾功能损害指标，利用 酶联免疫等方法检测血浆和肾组织血管转换酶活性及含量、血管紧张素