大肠杆菌（E.Coli）F-%2c41-黏附素基因原核表达及鸡抗F-%2c41-菌毛蛋白卵黄抗体的制备.pdf

Abstract adhesion Coil Escberichia 1．ExpressionofF4t geneofEnterotoxigenic The diarrheaisainfectionsdiseasecaused EschedchaColi．Withthe pi酣cts byEnterotoxigenic as adhesion themain adhesionWas F4I protein F41 studyobject，the geneexpressed． ofF4)adhesion (1)Analysis gene ThetotalDNAWasextractedfromETEC the WaS PCRwima strain,and by F41geneamplified contained sitesatthetwoterminals．andthe OfPCRwas pairofprimers specificenzyme product resultshowedthat：the adhesionis ofthe sequenced．The ofF41 length gene834bp，thehomologies nucleotide sequenceofF41werel00％comparingwithstrainsfromGenBank(M21788)． oftheadhesion F41 (2)Expression gene The PCR and vector WgTe thesame F41 expression purified product pET-28adigestedby enzymes tofrom clonesites．The Wasinsertinto then respectivelyparticular targetfragment pET-28aand transformedintoE．coli Wasextractedand DH5a,recombinant expressionplasmidpET-F(I trailsfo皿edintothe strainE．coliBL21andinducedIPTGfor results expression by expression．The showthat：recombinant Was constructedandtransformedinto expressionplasmidpET-28asuccessfully E．coli SDSshow adhesioncould inE．coil BL21；the