《Molecular cloning and function analysis of insulin-like growth factorbinding protein la in blunt snout bream （Megalobrama amblycephala）.》.pdf

ZoologicalResearch35(4)：300—306 DOI：10．13918j／．issn．2095-8137．2014．4．300 M olecularcloningandfunction analysisofinsulin-·likegrowthfactor- bindingproteinlainbluntsnoutbream (Megalobramaamblycephala) Yu．M eiTIAN，JieCHEN，YangTAO，Xia—YunJIANG,Shu．M ingZOU KeyLaboratoryofFreshwaterAquaticGeneticResources，ShanghaiOceanUniversity，Shanghai201306，China Abstract：Insulin．1ikegrowthfactor-bindingprotein1(IGFBP一1)，ahypoxia—inducedprotein，isamemberoftheIGFBPfamilythat regulatesvertebrate growt h anddevelopment．Inthisstudy,full—length IGFBP．1aeDNA wascloned from ahypoxia—sensitive Cyprinidaefishspecies，thebluntsnoutbream (Megalobramaamblycephala)．IGFBP—lawasexpressedinvariousorgansofadult bluntsnoutbrema ．includingstronglyintheliverandweaklyinthegonads．Underhypoxia，IGFBP—lamI A 1evelsincreased sharplyintheskin，liver,kidne spleen，intestineandhearttissuesofiuvenilebluntsnoutbream，butrecoveredtonormallevelsafter 24．hourexposuretonormaldissolvedoxygen．Inbluntsnoutbream embryos．IGFBP-lamRNA wasexpressedatverylow levelsat both fourand eighthourspost-fertilization．and strongly at1aterstages．Embryonicgrowth anddevelopmentratesdecreased significantlyinembryosinjectedwithIGFBP．1amRNA．TheaveragebodvlengthofIGFBP一1a-overexpressedembryoswas82．4％ ofmatOfthecontrolgroup．nadsomitenumbersdecreasedto85-2％．Thesefindingssuggestthathypoxia—inducedIGFBP—lamay inhibitrgowthinthisspeciesunderhypoxicconditions． Keywords：Megalobramaamblycephala；IGFBP—la；Hypoxia；Overexpression Thegrowthofvertebratesisprimarilyregulatedby grasscarp embryos，which suppressesthegrowth and theinsulin—likegrowthfactors(IGF)signalingpahtway developmentratesofgrasscapr embryos(Sunetal， (Bakeretal，1993：DuanXu，2005：ReindlSheridan， 2011)．Here，wedescribethemolecularcharacterization 2012、． Insulin—like growth factor-binding proteins andufnctionanalysisofIGFBP—lainbluntshoutbream (IGFBPs)，wiht molecularweightsof24x103-50x10， (Megalobr