《Purification and Photodynamic Bioactivity of Phycoerythrin and Phycocyanin from Porphyra yezoensis Ueda.》.pdf
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《Purification and Photodynamic Bioactivity of Phycoerythrin and Phycocyanin from Porphyra yezoensis Ueda.》.pdf
OceanUniv.China(OceanicandCoastalSeaResearch)
D0I10.1007/sl1802.014.2148一x
ISSN 1672-5182,201413(2):479-484
http.//www.ouc.edu.cn/xbywb/
E-mail:xbywb@ouc.edu.cn
PurificationandPhotodynamicBioactivityofPhycoerythrin
andPhycocyaninfrom PorphyrayezoensisUeda
CAIChuner),21W ANG Yuan ,LIChunxia4)GUO Ziye¨JIA Rui), WU W eining¨
, , , , ,
HU Yan¨andHEPeimin),),
.
1)AquacultureandLifeCollege,ShanghaiOceanUniversity,Shanghai201306,P.R.China
2)InstituteofMarineScience,ShanghaiOceanUniversiyt,Shanghai201306,P.R.China
3)LaboratoryofPharmacologyandPharmacodynamies,ShanghaiUniversityofTraditionalChineseMedicine,
Shanghai201203.P R.China
4)ShanghaiCPGuojianPharmaceuticalCo.,Ltd.,Shanghai201203,PR.China
(ReceivedSeptember4,2012;revisedOctober23,2012:acceptedSeptember29,2013)
@ OceanUniversityofChina.SciencePressandSpring-VerlagBerlinHeidelberg2014
Abstract Phycoerythrinandphycocyaninwerepurifiedfrom PorphyrayezoensisUedawiththeirbioactivitydeterminedinthis
study.Continuousprecipitationwithammonium sulfateatdifferentconcentrationsf10%,20%,40% and50%)increasedthepurity
(A564.A280)ofphycoerythrinto1.49,3.92foldoftherawextract(0.38)andthepurity( 15.A280)ofphycocyaninto0.70,3.33foldof
therawextract(O.21).Twomoretimesofchromatographywimhvdroxylapatitesfinallymadehtepurityofphycoerythrinandphy—
cocyaninreach5.50,14.47foldoftheraw exrtact,nad5.10。24.29foldoftherawexrtact,respectviely.Theyieldofhighpuriyt
phycoerythrinandphycocyaninwere0.21% nad0.09% ofdried yezoens blade.respectively.Thephotodynamiccytotoxicex.
perimentshowedthatbothphycoerythrinandphycocyanininhibitedthegrowthoflivertumorcellssignificantly.Itwasfoundthat
250mgL purifiedphycoerythrinandphycocyna ininhibitedthegrowt hofhepatocellularcarcinomacells24hafterlaser-irradiation
by80% and59%.respectively.and100mgL purifiedphycoerythrinandphycocyanininducedhteapoptosisOf31
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