《Purification and Photodynamic Bioactivity of Phycoerythrin and Phycocyanin from Porphyra yezoensis Ueda.》.pdf

《Purification and Photodynamic Bioactivity of Phycoerythrin and Phycocyanin from Porphyra yezoensis Ueda.》.pdf

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《Purification and Photodynamic Bioactivity of Phycoerythrin and Phycocyanin from Porphyra yezoensis Ueda.》.pdf

OceanUniv.China(OceanicandCoastalSeaResearch) D0I10.1007/sl1802.014.2148一x ISSN 1672-5182,201413(2):479-484 http.//www.ouc.edu.cn/xbywb/ E-mail:xbywb@ouc.edu.cn PurificationandPhotodynamicBioactivityofPhycoerythrin andPhycocyaninfrom PorphyrayezoensisUeda CAIChuner),21W ANG Yuan ,LIChunxia4)GUO Ziye¨JIA Rui), WU W eining¨ , , , , , HU Yan¨andHEPeimin),), . 1)AquacultureandLifeCollege,ShanghaiOceanUniversity,Shanghai201306,P.R.China 2)InstituteofMarineScience,ShanghaiOceanUniversiyt,Shanghai201306,P.R.China 3)LaboratoryofPharmacologyandPharmacodynamies,ShanghaiUniversityofTraditionalChineseMedicine, Shanghai201203.P R.China 4)ShanghaiCPGuojianPharmaceuticalCo.,Ltd.,Shanghai201203,PR.China (ReceivedSeptember4,2012;revisedOctober23,2012:acceptedSeptember29,2013) @ OceanUniversityofChina.SciencePressandSpring-VerlagBerlinHeidelberg2014 Abstract Phycoerythrinandphycocyaninwerepurifiedfrom PorphyrayezoensisUedawiththeirbioactivitydeterminedinthis study.Continuousprecipitationwithammonium sulfateatdifferentconcentrationsf10%,20%,40% and50%)increasedthepurity (A564.A280)ofphycoerythrinto1.49,3.92foldoftherawextract(0.38)andthepurity( 15.A280)ofphycocyaninto0.70,3.33foldof therawextract(O.21).Twomoretimesofchromatographywimhvdroxylapatitesfinallymadehtepurityofphycoerythrinandphy— cocyaninreach5.50,14.47foldoftheraw exrtact,nad5.10。24.29foldoftherawexrtact,respectviely.Theyieldofhighpuriyt phycoerythrinandphycocyaninwere0.21% nad0.09% ofdried yezoens blade.respectively.Thephotodynamiccytotoxicex. perimentshowedthatbothphycoerythrinandphycocyanininhibitedthegrowthoflivertumorcellssignificantly.Itwasfoundthat 250mgL purifiedphycoerythrinandphycocyna ininhibitedthegrowt hofhepatocellularcarcinomacells24hafterlaser-irradiation by80% and59%.respectively.and100mgL purifiedphycoerythrinandphycocyanininducedhteapoptosisOf31

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