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枫杨抗菌活性成分地研究.pdf

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目 录 中文摘要······························································1 英文摘要·····························································3 引 言··························································5 正 文·······························································7 第一部分 枫杨提取分离鉴定及抗菌活性筛选······························7 1.1 实验材料和试剂···················································7 1.1.1 材料及鉴定·············································7 1.1.2 菌种···················································7 1.1.3 试剂···················································7 1.1.4 仪器···················································7 1.2 化学成分预试验···········································8 1.2.1 水提取液〃···············································8 1.2.2 乙醇提取液··············································9 1.2.3石油醚提取液···················································10 1.2.4 化学成分小结··········································10 1.3 提取与分离定············································10 1.3.1 抗菌活性的测定方法·····································10 1.3.2 抗菌活性成分的分离纯化································12 1.3.2.1 提取·················································12 1.3.2.2.4 技术路线〃·········································15 1.4 结构鉴定················································17 1.4.1 化合物A 的结构解析·····································17 1.4.2 化合物B 的结构解析·····································2 1 1.4.3 化合物C 的结构解析·····································22 1.4.4 化合物D 的结构解析·····································26 1.4.5 化合物E 的结构解析·····································30 1.4.6 化合物F 的结构解析·····································37 1.4.7 化合物G 的结构解析·····································43 1.4.4 化合物H 的结构解析·····································48 1.5 碱溶酸沉法对抗菌活性成分的研究··························51 1.5.1 活性成分的筛选方法与追踪·······························51 1.6 活性成分提取分离及鉴定研究小结··························54 第二部分 枫杨的萘醌抗菌活性研究·····························55 2.1 实验材料············································

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