《PEG分级沉淀法分析天女木兰种子的低丰度蛋白 Polyethylene Glycol Fractionation Analysis of Low-Abundant Proteins in Magnolia sieboldii Seeds》.pdfVIP
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《PEG分级沉淀法分析天女木兰种子的低丰度蛋白 Polyethylene Glycol Fractionation Analysis of Low-Abundant Proteins in Magnolia sieboldii Seeds》.pdf
第49卷第11期 林 业 科 学 V01.49,No.11
SCIENTIASILVAESINICAE 01 3
2013年11月 Nov.,2
doi:10.11707/j.1001·7488
PEG分级沉淀法分析天女木兰种子的低丰度蛋白
陆秀君1’3 张晓林1’2’3 刘广林1 李天来2’3
(1.沈阳农业大学林学院沈阳110866;2.沈阳农业大学园艺学院沈阳110866;
3沈阳农业大学设施园艺省部共建教育部重点实验室 沈阳110866)
关键词: 天女木兰; 种子;蛋白质提取;PEG分级沉淀法
中图分类号:$718.43 文献标识码:A 文章编号:1001—7488(2013111—0189—06
FractionationofLow—Abundant
PolyethyleneGlycol Analysis
in Seeds
Proteins sieboldii
Magnolia
Lu Xiaolinl2·3Liu LiTianlai2,3
Xiujunl·3Zhang Guanglinl
110866;
(1.CollegeofForestry,ShenyangAgriculturalUniversityShenyang
1
2.Col如geofHorticulture,ShenyangAgriculturalUniversityShenyang10866;
ProtectedHorticulture 1
Laboratory Ministry 10866)
3.Key of of ofEducation,ShenyangAgriculturalUniversityShenyang
Abstract: seedsstore amountof thatwouldinterferewithdeterminationoflow
Magnolia.sieboldiilarge proteins
abundance the this leaf extractionmethodwas
proteinsby electrophoresispattern.Instudy,aplantprotein
RubiscoPEG.ThePEGfraetionationmethodwas withTCA·aeetone
is,enrichinghigh—abundanceprotein by compared
extractme
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