《基于硒蛋白生物合成机制的硒生物检测器 Biological Selenium Detector Based on Mechanism of Selenoprotein Biosynthesis in Prokaryotes》.pdfVIP

ofAnhui 责任编辑朱琼琼责任校对况玲玲 安徽农业科学，Journal Ash．Sci．2013，41(11)：4729—4734 基于硒蛋白生物合成机制的硒生物检测器 邱洋松，孙婧，孙爱，邬丹妮，张冰倩，马建辉，徐丽珊，孙梅好4 (浙江师范大学化学与生命科学学院，浙江金华321004) 摘要硒是许多生物的必需微量元素，与人类健康关系密切。硒在自然界中分布的不平衡导致不同地区的硒含量差异较大，易引起硒 insertionse— 补充目前常用硒含量测定方法提供了可能。该研究利用原核载体构建硒代半胱氨酸插入所需茎环结构(Selenocysteine 具有较好的线性关系。此结果表明，试验已初步构建了大肠杆菌硒生物检测器，为利用此方法进一步分析测定环境及生物样品中的硒 含量奠定了基础。 关键词硒蛋白：硒代半胱氨酸；生物检测器；茎环结构 中图分类号S188；Q599文献标识码A 文章编号0517—6611(2013)11—04729—06 SeleniumDetectorBasedonMechanismof in Biological SelenoproteinBiosynthesisProkaryotes etal of andLifo Normal QIUYang-song(SchoolChemistryScience，ZhejiangUniversity，Jinhua，Zhejiang321004) Abstract traceessentialelementsfor crucialtohumanhealth．Imbalanceofseleniumdistributioni11nature Selenium．one manyorganisms．iS inlocal or wouldresultthemarkedvariationenvironmentalseleniumlevelswhichwouldleadtotheselenosisselenium elucidation deftciency．The of for of in madeit fortIleconstructionof selenium pathwaysbiosynthesisselenocysteine(Sec)andselenoproteinsprokaryotespossible biological detectorwhichwould thecurrentselenium insertion fortIIeSec supplement measuringmethods．Selenocysteinesequence(SECIS)iSnecessary its nucleotideshadbeenfoundinfluencedthe forSec the ac— insertion，andpreceding efficiencyinsertion．Byvaryingprecedingnucleotides，high curateandefficientsEClswerescreenedbasedontheeffectsontranslationof which was detectedbacterialanti．aln— 13．1actmaseactivityeasily by