《5-氟脲嘧啶腹腔化疗对鼠胃肿瘤细胞凋亡的实验研究》.pdf

256 塞丝匡堂至塑!生箜垫鲞笙i翅 5一氟脲嘧啶腹腔化疗对鼠胃肿瘤细胞凋亡的实验研究 潘晋方 于庆生卢业才 [摘要]目的通过对种植性胃肿瘤鼠腹腔注射5一氟脲嘧啶(5一Fu)化疗，探讨5一Fu腹腔化疗对鼠胃肿瘤细 组，生理盐水(对照组)和5一Fu组。接种后七天，对照组每只每天腹腔注射生理盐水40 ml／kg，5一Fu组每只每天腹腔 20 注入5一Fums／kg，两组均连用5天，于化疗结束后第七天每组处死lO只。观察：肿瘤体积(肿瘤体积抑制率)，Tdt介 下10只观察生存时问，并计算生存时问延长率。结果生理盐水组动物于接种后2周出现食量明显减少。活动少，倦怠 状，对外界刺激反应迟钝，逐渐消瘦，甚至腹水形成，而5一Fu组接种后4周才出现，与生理盐水组相比，5一Fu组鼠胃肿 7％(P0．05)。肿瘤细胞凋亡面积增大，光密度明显升高。结论5一Fu有明显的诱导胃肿瘤细胞分化与凋亡作用。 [关键词]胃肿瘤；5一氟尿嘧啶；腹腔化疗；细胞凋亡 on to tumorin ratswith5一FU Experimentalstudyapoptosisimplantedgastric byintrapedtonealchemotherapy Pan Yecai Jinfang，Yu Qingsheng，Lu General First toAnhui TraditionalChinese 230031 Departmentof Surgery，theHospitalAffiliated Collegeof Medicine，Hefei To effecton to GastricTumorinRatswith [Abstract]Objectivestudy apoptosisImplanted 40Wistarratswere withthe cellline tumor tumor IntraperitonealChemotherapy(IPC)．Methodsimplantedgastric malignant Walker一256andthendivided into2 and5一Fu theendofthe randomlygroups：0．9％sodiumchloride(NS)groupgroup．At 7th after ratsineach wereexecutedtotesttumorvolume．Theweremeasuredterminal day IPC，10 group apoptosis by deoxynu- transferase mediatednickand itsareaand leotidyl labeling(TUNEL)methods，anddensityprocessedautomatically byoptical withtheIMScell restoftheratswere