玻璃体切除术后晶状体上皮细胞变化及茶多酚眼用凝胶干预作用实验地研究.pdfVIP

培养条件下的上皮细胞线粒体膜电位降低；而茶多酚眼用凝胶干预组细胞线粒体膜电 位升高。6. 经免疫荧光检测，在 BSS 、硅油、O 、C F 培养条件下晶状体上皮细胞 2 3 8 中内质网的阳性率明显降低，而应用茶多酚眼用凝胶干预后内质网的阳性率明显升 高。7. 经免疫组化检测，在 BSS 、硅油、O 、C F 培养条件下晶状体上皮细胞中 2 3 8 Caspases-3 表达明显，而应用茶多酚眼用凝胶干预后 Caspases-3 表达明显降低。 结论：玻璃体切除术后微环境的改变引发晶状体上皮细胞的凋亡，上皮细胞的凋 亡是玻切术后并发性白内障发生的原因之一，而茶多酚眼用凝胶能够有效抑制上皮细 胞线粒体活性氧水平，保护线粒体膜电位及内质网的功能，降低细胞凋亡过程中最重 要的终末执行酶Caspases-3表达水平，从而抑制晶状体上皮细胞凋亡。保护晶状体上 皮细胞免受损害，维持其结构和功能的正常，从而可能延缓玻切术后并发性白内障的 发生。 关键词 玻璃体切除术；晶状体上皮细胞；细胞凋亡；茶多酚眼用凝胶； Empirical Study on the Effects of Vitrectomy and the Intervene of Tea Polyphenols Ophthalmic Gel on the Lens Epithelial Cells Speciality： Ophthalmology Tutor： Prof. Bi Hongsheng Author ： ZhangLi Abstract Objective ：To observe ROS expression in mitochondria ，membrane potential ， Caspases-3 and endoplasmic reticulum of rabbit lens epithelial cells cultured after vitrectomy and the changes of them after the intervention of tea polyphenols ophthalmic gel.To study the causes and effects of these changes，and to further explore the complicated cataract and tea polyphenols ophthalmic gel，s curative effect. Methods ：1. Rabbit lens epithelial cells were cultured in vitro in different special selective medium made from BSS，silicone oil，O ，C F concentration to simulate the 2 3 8 vitrectomy in vivo.The morphous changes of the cells were then observed when they were intervened in different concentration of tea polyphenols ophthalmic gel.2. Prepare medium with final concentrations of 1000μg/ml 、100μg/ml 、10μg/ml 、1μg/ml 、0μg/ml tea polyphenols ophthalmic gel to clture rabbit lens epithelial cells in vitro， and use CCK-8 to choo