《5-羟色胺在野百合碱引起大鼠肺动脉高压发病中的作用》.pdf

中国病理生理杂志990525 中国病理生理杂志 CHINESE JOURNAL OF PATHOPHYSIOLOGY 1999年 第15卷 第5期 vol.15 No.5 1999 5-羟色胺在野百合碱引起大鼠肺动脉 高压发病中的作用 张福琴　李志超　赵德　黄威权　孙本韬 　　摘　要　目的：探讨5-HT在MCT性肺动脉高压中的作用。方法：用免疫组化方法 和图象分析技术，测定肺组织中5-HT及其受体的变化；采用MTT法和测微网及钙荧光 指示剂Fura 2/Am测定培养的肺动脉平滑肌细胞生长情况和收缩功能以及细胞内游离 2+浓度。结果：发现MCT引起大鼠肺动脉高压时肺组织中5-HT(127 494±20 736/ Ca 2 2 mm)及其受体(14 624±3 010/mm)阳性细胞数目比对照组(3 720±1 018, 3 430±821/ 2 mm)的均显著增加(P＜0.01)。5-HT能使培养的肺动脉平滑肌细胞增生和收缩这种作用 2+ 内流有关。结论：5-HT的收缩血管作用和促血管平滑肌细胞增殖作用 与其引起的Ca 在MCT性肺动脉高压的发病机制中可能具有重要的意义。 　　主题词　血清素；高血压；肺动脉；野百合碱；大鼠 The role of 5-hydroxypytamine in monocrotaline-induced pulmonary hypertension 1 2 2 3 4 ZHANG Fu-Qin , LI Zhi-Chao ,ZHAO De-Hua , HUANG Wei-Quan , SUN Ben-Tao Department of Pathophysiology, 2 Pharmacology, 3 Histology and Embrodylogy, Fouth Military Medical University , Xian (710032),4 Department of Electron Microscope, Shanxi Medical College, Taiyuan 　　Abstract AIM and METHODS: To explore the effect of 5-hydroxytryptamine (5-HT) in monocrotaline(MCT)-induced pulmonary hypertension, 5-HT and 5-HT receptor ( 5- HTR) in pulmonary tissue was observed by immohistochemistry and image analysis system. Cellular growth, constrictions function and free Ca2+ in cultured pulmonary artery smooth muscle cells were measured by the methods of MCT, morphometry and calcium fluorescent probe Fura 2-Am. RESULTS ：The numbers of 5-HT(127 494±20 736/mm2) and 5-HTR (14 624±3 010/mm2) positive cells in pulmonary tissue induced by MCT were increased compared with control (3720±1018, 3430±821/mm2). 5-HT could induce both cellular growth and contraction of pulmonary arterial smooth