蟾酥中有效成分群抗肿瘤配伍地研究.pdf

Stu dy on th e Rat io of A ct ive Ing red ient s o n A n t i- t um o r E f f e c t in V e n e n u m B u f o n is Sp ec ia lity : Pharm a cy of TCM A u t h o r : G a o Y a n T u t o r : L in G u i- t a o A b s t r a c t Ob ject ive : M ak e a stu dy of th e a ct ive in gr ed ien t s th at h av e an t i- tum or effect in V en enum Bu fon is, scr een ed ou t of th e m o st appr opr iat e pr opor t ion of th e thr ee toa d p o ison dru g s. M ethod s : Th e m eth od of M TT an d w r ith ing w ith a ce t ic a c id w er e u sed to determ in e th e cell grow th inh ib it ion on BEL- 7402 h epatom a ce ll lin es an d th e ana lge sic effect of th e m ice of Bu fa lin , c in ibu fa g in an d r esibu fogen in a t d iff er en t con cen tr at ion an d d iff er en t t im e . an d to exp lor e th e r o le of r esibu fogen in to Bu fa lin ， c in ibu fa g in . U lt im a te ly , U s in g or th o gon a l te st m etho d S tu d ied com pat ib ility of th e thr ee toa ds p o ison dru g s ab ou t th e an t i- tum or eff ect an d a na lge s ic eff ect s. Resu lts : ① The IC50 va lues of BF 、CBG 、RBG in th e ro le of cells after 24h are： 1. 560X 10~\ 1. 393X 10_6、8. 128 X 103, BF 、CBG 、RBG can sign ificantly inh ib it th e pr o lifer at ion of BEL- 7402 human h epatom a cells, thr ee toa ds p o ison dru g s hav e a c lear do se an d t im e ef fect r e la t ion sh ip on h ep a tom a ce lls, an d inh ib itory effect in cr ea sed W ith th e ex ten s ion o f t im e or th e in cr ea se of con cen tr a t ion . In th e m ou se w r ith in g ana lge s ic exp er im en t s, th e inh ib it ion of them sign ificantly increase w ith the increase of concentration. ② c om b in a t io n o f tw o o f t h e d if f er e n t r a t io o