连锁关联分析在疾病的研究中的探讨.pptVIP

宝山壁画 宝山壁画是引人注目的昂贵文物。此壁画发现于阿鲁科尔沁旗东沙布乡境内。1994年列为“全国十大考古新发现”之一。宝山壁画中最引人注目的是《杨贵妃教鹦鹉图》。该画高0.7米、宽2.3米，用于笔重彩绘制，最突出的表现了 晚唐风格。唐代擅长绘贵妇仕女的大师周昉绘制了《杨贵妃教鹦鹉图》，不仅享誉中原，而且还影响全国各地。发现于阿旗宝山古墓里的这幅画，就是契丹人聘请中原画家按照周氏风格绘制的， 技法深得周氏画风的真传。在唐人真迹稀如星风的今天，能够从中完整了解唐代人物画的杰出成就，堪称美术史研究的辛事。这幅壁画现今保存在阿鲁科尔沁旗博物馆，历经千年，恍如新绘，是该馆的镇馆之宝。 欢迎大家观看！ * * * To date there have been over 5300 publications using a GWAS approach to explore the contribution of genetic variants to diseases and traits of interest. And this number has grown dramatically over the past couple of years – from just a handful of papers in the mid-2000’s to over 200 in 2009 alone. * * * Illumina’s genotyping assay is called the GoldenGate Assay It is a high specificity extension and ligation assay that allows the simultaneous analysis of over 1500 loci in a single reaction and uses the UNIVERSAL Sentrix arrays by incorporating into the reaction products a unique 地址 sequence for each locus being interrogated that is matched to a specific bead type’s illumiCode One of the most important features of the assay is that it uses genomic DNA and not amplified DNA as the genotyping target For each locus being interrogated 3 specific oligos are used 2 allele specific oligos (ASOs) , both of which have a sequence region that is a perfect complement to the genomic region directly adjacent to the target SNP site but that differ in their 3 prime base such that they only match one of the 2 alleles at the site, and a second region that acts as a universal primer site for the subsequent amplification reaction A third locus specific oligo (LSO) hybridizes between 1 to 20 bases downstream of the target SNP site through a complementary sequence. Mention benefit of flexible LSO placement is avoidance of hybridizing over other SNPs in close proximity The LSO also contains two other components, a unique illumiCode sequence that perfectly matches an illumiCode oligo on an array bead and a third universal primer sequence. After oligo hybridization, a po