仪器分析-高效液相色谱(HPLC)-华中农业大学课件.ppt

* * High Performance Liquid Chromatography, HPLC Chapter Eleven Chromatography HPLC GC Section One Introduction Developing History of HPLC The Difference Between HPLC and C lassical LC The Comparison Between HPLC and GC Developing History of HPLC HPLC was developed raidly in 1970’s. Based on the foundation of classical LC. Meanwhile, adopting the theory of GC. The earliest LC was found in 1906 by Michael Tswett. Leaf in mineral ether Mineral ether (Mobile phase) ? ?  Chlorophyll ａ Column Chlorophyll ｂ Carotenol Calcium carbonate Provitamin A （Stationery phase） ?  Chromatogram ? ? Procedure： Elution ? Method： Chromatography The Difference Between HPLC and Classical LC Employing high pressure pump to apply high pressure ( in the range of 150~350x105 Pa) To reduce the resistance of liquid in column. To improve the transmission and analysis velocity. B. Employing new stationary phase-chemially bonded stationary phase a. To improve the separation efficiency eg. N=30000/m C. Employing Detectors with High Sensitivity Ultraviolet photometric detector; Fluorescence detector; Differential refractive index detector; Electrical conductivity detector eg. UV-detector DL 10-9g Fluorescence- detector DL 10-11g D. High Automatic Degree with Computer ( Chemi-station) Summarize: “Four High”—High pressure High velocity High efficiency High sensitivity The Comparison Between HPLC and GC Similarity The