白花丹参化学成分及其保护血管内皮细胞地研究.pdf

The chemical composition and their protection of blood vessel endothelial cell of Salvia miltiorrhiza f.alba Specialty：Pharmacy of TCM Author ：Wang Peng Tutor：Professor Gou Qing-mei ＆Zhou Feng-qin Abstract Objective：In order to provide the scientific basis on curing thromboangiitis obliterans of Salvia miltiorrhiza f.alba(SAM), carry on the solvent extraction and various chromatographic methods to separate and identify its chemical composition, whose protective function to the blood vessel endothelial cell was discussed. Methods ：Using the modern separation methods (pocket resin, silica gel and gelatin column chromatograph), separate and purify the fat-soluble and the water-soluble chemical composition of SAM, and identify their structure by IR, MS, 1H-NMR and 13C-NMR. carries on the appraisal to the obtained compound structure.Divided human umbilical vascular endothelial cell(HUVEC) in vitro into the normal control group, the LPS damage group, the SAM protection group; stained cells with acridinorange, and then observed cells under laser scanning confocal microcopy; stained cells with PI, and ananlyzed the cell cycle distribution with flow-cytometric The activities of glutathione peroxidase (GPH-PX) and Superoxide Dismutase (SOD) as well as maleic dialdehyde (MDA) level in cells were measured by biochemistry reagent box. Results ：11 compounds were obtained from chloroform extraction of SMA, and 7 compounds were identified by various genealogy technology, including tanshinone ⅡA,cryptotanshinone,15,16-dihydrotanshinone,tanshinone, β-sitostenrol, danshixinkun B, danshensu. The fat-soluble ingredient and component of SMA had no protective fu