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Cellular response to titanium discs coated with polyelectrolyte multilayer films.pdf
InternationalJournalofMinerals,MetallurgyandMaterials
Volume21,Number9,September2014,Page925
D01:10.1O07/s12613-014-0991-9
Cellularresponsetotitanium discscoatedwithpolyelectrolytemultilayerfilms
JingZhann Qiao-jieLuo,YingHuang2),andXiao—dongLi2’
,
1)DepartmentofStomatology,SirRunRunShawHospital,CollegeofMedicine,ZhejiangUniversity,Hangzhou310016,China
2)DpeartmnetofOralandMaxillofaeialS~gery,theAffiliatedStomatologyHospital,CollegeofMedicine,ZhejiangUniversity,Hangzhou310031,China
(Received:26April2014;revised:22May2014;accepted:28May2014)
Abstract:n蟛purposeofthisstudywastoinvestigatetheeffectsofpolyelectrolytemultilayer(PEM)coatingsonhtebiologicalbehaviorof
titanium (Ti)substrates.CollagentypeIh/yaluronicacid(CoVHh)andchitosan/hyaluronicacid(Ch~I-IA)multilayerPEM coatingswerein·
troducedontoTisubs~amsusniglayer-by.1ayerassembly.Contactnaglenistrumentsandquartzcrystalmicrobalancewereusedforfilm
characterization.111eresultsobtaniedshowedthatbom Co andChi/HA surfaceshadhi hydrophilicitynadpromotedeelladhesionin
MC3T3.E1pre.osteoblastnadhmu na gingivalfibroblastcells.Inaddition.htesynthesisoffunction.relatedproteinsna dgeneexpression
levelsniboht MC3T3.E1nadfibroblastcellswashigherofr血eCo coatingcomparedwihthteCh coatnig.nidicatingbeaercellu—
lraresponsetohteCo coatnig.
Keywords:titna ium ;surfacemodification;polyelectrolytes;multilayerfilms;cellulra response
chitosallnad cOllagen havebeen extensively studiedofr
1.Introduction hteirapplicationsniwoundhealnig[3 ],asscaffoldsofr
tissueengnieering[5-6],nadbiomedicalmaterials.Immo—
nlebenefitsofsurfacemodificationareevidentfrom the bilziationofcollagenorchitosna ontohtesurfacesoftim-
applicationsofclinicalimplants.Controlledsurfac
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