Cellular response to titanium discs coated with polyelectrolyte multilayer films.pdfVIP

Cellular response to titanium discs coated with polyelectrolyte multilayer films.pdf

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Cellular response to titanium discs coated with polyelectrolyte multilayer films.pdf

InternationalJournalofMinerals,MetallurgyandMaterials Volume21,Number9,September2014,Page925 D01:10.1O07/s12613-014-0991-9 Cellularresponsetotitanium discscoatedwithpolyelectrolytemultilayerfilms JingZhann Qiao-jieLuo,YingHuang2),andXiao—dongLi2’ , 1)DepartmentofStomatology,SirRunRunShawHospital,CollegeofMedicine,ZhejiangUniversity,Hangzhou310016,China 2)DpeartmnetofOralandMaxillofaeialS~gery,theAffiliatedStomatologyHospital,CollegeofMedicine,ZhejiangUniversity,Hangzhou310031,China (Received:26April2014;revised:22May2014;accepted:28May2014) Abstract:n蟛purposeofthisstudywastoinvestigatetheeffectsofpolyelectrolytemultilayer(PEM)coatingsonhtebiologicalbehaviorof titanium (Ti)substrates.CollagentypeIh/yaluronicacid(CoVHh)andchitosan/hyaluronicacid(Ch~I-IA)multilayerPEM coatingswerein· troducedontoTisubs~amsusniglayer-by.1ayerassembly.Contactnaglenistrumentsandquartzcrystalmicrobalancewereusedforfilm characterization.111eresultsobtaniedshowedthatbom Co andChi/HA surfaceshadhi hydrophilicitynadpromotedeelladhesionin MC3T3.E1pre.osteoblastnadhmu na gingivalfibroblastcells.Inaddition.htesynthesisoffunction.relatedproteinsna dgeneexpression levelsniboht MC3T3.E1nadfibroblastcellswashigherofr血eCo coatingcomparedwihthteCh coatnig.nidicatingbeaercellu— lraresponsetohteCo coatnig. Keywords:titna ium ;surfacemodification;polyelectrolytes;multilayerfilms;cellulra response chitosallnad cOllagen havebeen extensively studiedofr 1.Introduction hteirapplicationsniwoundhealnig[3 ],asscaffoldsofr tissueengnieering[5-6],nadbiomedicalmaterials.Immo— nlebenefitsofsurfacemodificationareevidentfrom the bilziationofcollagenorchitosna ontohtesurfacesoftim- applicationsofclinicalimplants.Controlledsurfac

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