水稻同源异型域转录因子OsHox9的反向遗传学分析.doc

水稻同源异型域转录因子OsHox９的反向遗传学分析 艾丽萍　申奥　高志超　李政龙　孙琼琳　栾维江 (天津师范大学生命科学学院/天津市动植物抗性重点实验室,天津３００３８７;通讯联系人,EＧmail:lwjzsq＠１６３．com) ReverseGeneticsAnalysisoftheTranscriptionFactorOsHox９,aMemberof HomeoboxFamily,inRice AILiＧping,SHENAo,GAOZhiＧchao,LIZhengＧlong,SUNQiongＧlin,LUANWeiＧjiang (CollegeofLifeScience,TianjinNormalUniversity/TianjinKeyLaboratoryofAnimalandPlantResistance,Tianjin３００３８７, China;Correspondingauthor,EＧmail:lwjzsq＠１６３．com) AILiping,SHENAo,GAOZhichao,etal．ReversegeneticsanalysisofthetranscriptionfactorOsHox９,amemberof homeoboxfamily,inrice．ChinJRiceSci,２０１４,２８(３):２２３Ｇ２２８． Abstract:Thehomeoboxtranscriptionfactorsplayimportantrolesinthegrowthanddevelopmentoftheplants, involvedinregulationofcelldifferentiation,thearchitectureofmorphogenesisandresponsetoenvironmentalsignalsin plants．Tostudythefunctionofthesetranscriptionfactorsinrice,weconstructedRNAivectorsofOsHox９and analyzedthefunctionofOsHox９bythereversegeneticsmethod．Theresultsshowedthattheheightandtillernumber ofRNAitransgenicplantsweredecreasedincomparisonwithwildtypeplants．RealＧtimePCRanalysisshowedthat OsHox９expressionlevelwasdownregulatedintransgenicplantswithphenotype．However,theexpressionlevelof OsHox９intransgenicplantswithoutphenotypewassimilartowildtypeplants,suggestingthatthephenotypesof transgenicplantswerecausedbyRNAieffects．Inaddition,wealsoanalyzedtheexpressionpatternofOsHox９in differentorgansofrice．TheresultsshowedthatOsHox９wasexpressedindifferentorgans,especiallydisplayedhigh expressionlevelinstemapicalmeristemsandyoungpanicles．ToinvestigatethesubcellularlocalizationofOsHox９,we constructedthefusedvector３５S::OsHox９ＧGFPtointroduceintoleavesoftobacco．Thetransientexpressionresult showedOsHox９waslocalizedonthecellmembrane． Keywords:rice;OsHox９;RNAi;expressionanalysis 艾丽萍,申奥,高志超,等．水稻同源异型域转录因子OsHox９的反向遗传学分析．中国水稻科学,２０１４,２８(３): ２２３Ｇ２２８． 摘　要:同源异型域(Homeobox,HB)转录因子对于植物的生长发育具有重要的调控作用,主要涉及细胞分化、形态建成、内 外环境信号应答等多个方面.为了研究该转录因子家族成员在水稻中的功能,构建了该家族成员OsHox９基因的RNAi表 达载体,利用反向遗传方法分析该基因的功能.与野生型对照植株相比,RNAi转基因植株株高变矮,分蘖数减少.实时 PC